Molecular Approaches to Diagnosing and Managing Infectious Diseases: Practicality and Costs

Molecular approaches to diagnosing and managing infectious diseases: practicality and costs.

As molecular techniques for identifying and detecting microorganisms in the clinical microbiology laboratory have become routine, questions about the cost of these techniques and their contribution to patient care need to be addressed. Molecular diagnosis is most appropriate for infectious agents that are difficult to detect, identify, or test for susceptibility in a timely fashion with convent...

Telemedicine application in diagnosing and managing heart diseases in neonates and children: A systematized review

Molecular Genomic Approaches to Infectious Diseases in Resource-Limited Settings

Only half a century after the landmark discovery of the double helix structure of DNA, the human genome was sequenced and a new era of biomedical research was ushered in [1]. Parallel advances in comparative genomics, genetics, highthroughput biochemical techniques, and bioinformatics have provided researchers in wealthy nations with a repertoire of tools to analyze the sequence and functions o...

Diagnosing infectious porcine diseases using immunohistochemistry

This review describes the foundations, methodology, applications, advantages, and disadvantages of immunohistochemistry used for the diagnosis of swine infectious disease. Immunohistochemistry (IHC) relies on the binding of immunoglobulins to antigens present in tissues. Numerous proteins from infectious agents (viruses, bacteria, parasites, fungi) can be detected with this technique. The antig...

Diagnosing infectious diseases using in situ hybridization

This note describes the foundations, methodology, principles, advantages and disadvantages of using in situ hybridization to diagnose swine infectious diseases. In situ hybridization (ISH) relies on the detection of complementary sequences of nucleic acids present in the tissue using labeled nucleic acid sequences (probes) specific for the infectious organism. Hybridization occurs between compl...